Achieving High Throughput Repositories for Biomedical
Germplasm Preservation Workshop—April 10, 2007
| 8–8:30 a.m. |
Registration and Continental Breakfast |
| 8:30–8:40 a.m. |
Welcome
Franziska Grieder, D.V.M., Ph.D., National
Center for Research Resources (NCRR) |
| 8:40–8:50 a.m. |
Charge to the Workshop Participants
Louise Ramm,
Ph.D., NCRR |
| 8:50–9:00 a.m. |
Definition of High Throughput Repositories
Moderator:
William F. Rall, Ph.D., NCRR |
| 9:00–10:30 a.m. |
Status of Germplasm Cryopreservation by Species and Germplasm
Format
Moderator: Kent Lloyd, D.V.M., Ph.D., University
of California, Davis
Current Status of the Achieving
High Throughput Repositories for Mouse and Rat Germplasm Preservation
Presenter: John Critser, Ph.D., University of Missouri,
Columbia
High Throughput Preservation of Swine
Germplasm
Presenter: Phillip Purdy, Ph.D., U.S. Department
of Agriculture (USDA)
Preservation of Germplasm
of Nonhuman Primates
Presenter: Catherine VandeVoort,
Ph.D., University of California, Davis
Current Status
of Cryopreservation in Aquatic Species
Presenter: Terrence
Tiersch, Ph.D., Louisiana State University Agricultural Center
|
| 10:30–10:50 a.m. |
Break |
| 10:50 a.m.–12 p.m. |
Examples of Equipment and Processes for High Throughput Repositories
Moderator: Terrence Tiersch, Ph.D.
Cryo Bio
System
Presenter: Brad Belstra, Ph.D., IMV International
Corporation
State-of-the-Art Research and Development
for Maximizing Germplasm Preservation
Presenter: John
Dobrinsky, Ph.D., Minitube International Center for Biotechnology
The National Animal Germplasm Program's Development
and Use of High Throughput Processes
Presenter: Harvey
Blackburn, Ph.D., USDA |
| 12–1 p.m. |
Lunch (on your own) |
| 1–2:30 p.m. |
Panel Discussion: Cryopreservation Methods and Optimization
of Protocols
Moderator: George E. Seidel, Ph.D., Colorado
State University
Fundamental (Mechanistic) and
Empirical Methods: Fundamental Approaches
Mechanistic
Presenter: John Critser, Ph.D.
Empirical
Presenter: Stanley Leibo, Ph.D., University of New Orleans
Chilling Injury and Cold Shock
Presenter: Stanley
Leibo, Ph.D. |
| 2:30–2:50 p.m. |
Afternoon Break |
| 2:50–5 p.m. |
Summary of Evaporative Drying of Mouse Sperm
Presenters:
Mehmet Toner, Ph.D., Massachusetts General Hospital; and Kent Lloyd,
D.V.M., Ph.D.
Ultra-Rapid Cooling Using Oscillating
Heat Pipe, Nanofluid, and Microfabrication Techniques
Presenter:
John Critser, Ph.D.
Improving Mouse Sperm Cryopreservation
Presenter: Robert Taft, Ph.D., The Jackson Laboratory |
| 5 p.m. |
Meeting Adjourns |
| 8–8:30 a.m. |
Continental Breakfast |
| 8:30–10:40 a.m. |
Panel Discussion: Physiological, Reproduction, and Disease
Considerations
Moderator: Thomas Kuehl, Ph.D., Scott
and White Memorial Hospital
Choice of Germplasm
Formats
Presenters: Kent Lloyd, D.V.M., Ph.D., and Phillip
Purdy, Ph.D.
Improving Gamete and Embryo QualityPresenters:
James Graham, Ph.D., Colorado State University; and George E. Seidel,
Ph.D. |
| 8:40–8:50 a.m. |
Charge to the Workshop Participants
Louise Ramm,
Ph.D., NCRR |
| 8:50–9:00 a.m. |
Definition of High Throughput Repositories
Moderator:
William F. Rall, Ph.D., NCRR |
| 9:00–10:30 a.m. |
Status of Germplasm Cryopreservation by Species and Germplasm
Format
Moderator: Kent Lloyd, D.V.M., Ph.D., University
of California, Davis
Current Status of the Achieving
High Throughput Repositories for Mouse and Rat Germplasm Preservation
Presenter: John Critser, Ph.D., University of Missouri,
Columbia
High Throughput Preservation of Swine
Germplasm
Presenter: Phillip Purdy, Ph.D., U.S. Department
of Agriculture (USDA)
Preservation of Germplasm
of Nonhuman Primates
Presenter: Catherine VandeVoort,
Ph.D., University of California, Davis
Current Status
of Cryopreservation in Aquatic Species
Presenter: Terrence
Tiersch, Ph.D., Louisiana State University Agricultural Center
|
| 10:30–11 a.m. |
Break |
| 11 a.m.–12 p.m. |
Biosecurity for High Throughput Cryopreservation
Presenter:
Terrence Tiersch, Ph.D. |
| 12–1 p.m. |
Lunch (on your own) |
| 1–2:30 p.m. |
Guidelines for Health Testing
Presenter: Lela Riley,
Ph.D., University of Missouri
Epigenetics
Presenter: George Seidel, Ph.D. |
| 2:30–3 p.m. |
Break |
| 3–4 p.m. |
Group Discussion: Formation of a Consensus
Moderators:
Advisory Committee Members |
| 4 p.m. |
Meeting Adjourns |